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Heterologous Expression of a Gibberellin 2-Oxidase Gene from Arabidopsis thaliana Enhanced the Photosynthesis Capacity in Brassica napus L.
Authors:Bo Zhou  Dan Peng  Jianzhong Lin  Xingqun Huang  Wusheng Peng  Reqing He  Ming Guo  Dongying Tang  Xiaoying Zhao  Xuanming Liu
Affiliation:(1) Bioenergy and Biomaterial Research Center, Collage of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, Hunan, China;(2) Academy of Seed Industry of Hunan Yahua, Changsha, 410001, Hunan, China
Abstract:Gibberellins (GAs) are endogenous hormones that play an important role in regulating plant stature by increasing cell division and promoting seed germination. The GA2-oxidase gene from Arabidopsis thaliana (AtGA2ox8) was introduced into Brassica napus L. by Agrobacterium-mediated floral-dip transformation with the aim of decreasing the amount of bioactive GA and hence reduced the plant height. As anticipated, the transgenic plant exhibited dwarf phenotype. Importantly, compared with the wild type, the transgenic plants had delayed the seed germination, increased the chlorophyll content (28.7–36.3%) and photosynthesis capacity (14.3–18.7%) in a single leaf. At the same time, the photosynthesis capacity of the whole plants was significantly enhanced (35.7–48.6%) due to the extra leaves and branches.
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