Structure of an acidic O-specific polysaccharide of the bacterium Providencia alcalifaciens O7 |
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Authors: | Bystrova O V Zatonskii G V Borisova S A Kocharova N A Shashkov A S Knirel Y A Kholodkova E V Stanislavskii E S |
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Institution: | Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia. knirel@ioc.ac.ru |
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Abstract: | An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Providencia alcalifaciens O7 and purified by gel chromatography followed by anion-exchange chromatography. On the basis of full acid hydrolysis, methylation, carboxyl reduction, selective cleavage with anhydrous hydrogen fluoride, and 1H- and 13C-NMR spectroscopy, including two-dimensional 1H,1H homonuclear and H-detected 1H,13C heteronuclear correlation spectroscopy and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: figure], where Rhap2Ac is 2-O-acetylrhamnopyranose. |
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