Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries |
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| Authors: | Nielsen Jeppe L Schramm Andreas Bernhard Anne E van den Engh Gerrit J Stahl David A |
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| Affiliation: | Department of Civil and Environmental Engineering, University of Washington, 302 More Hall, Box 352700, Seattle, WA 98195-2700, USA. |
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| Abstract: | A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes. |
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