Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana |
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| Authors: | Sangdun Choi Robert A Creelman John E Mullet Rod A Wing |
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| Institution: | (1) Crop Biotechnology Center Texas A&M University, 77843 College Station, TX, USA |
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| Abstract: | We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54).
Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability
of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all
probes hybridized to at least one (and in most cases three) BAC clones in the library. |
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