Preparation of lipophilic alkyl (hydroxy)benzoates by solvent-free lipase-catalyzed esterification and transesterification |
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Authors: | K Vosmann B Wiege P Weitkamp N Weber |
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Institution: | (1) Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, People’s Republic of China |
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Abstract: | Agal-fermentation-based microbio-diesel production was realized through high-cell-density fermentation of Chlorella protothecoides and efficient transesterification process. Cell density achieved was 16.8 g l−1 in 184 h and 51.2 g l−1 in 167 h in a 5-l bioreactor by performing preliminary and improved fed-batch culture strategy, respectively. The lipid content
was 57.8, 55.2, and 50.3% of cell dry weight from batch, primary, and improved fed-batch culture in 5-l bioreactor. Transesterification
was catalyzed by immobilized lipase, and the conversion rate reached up to 98%. The properties of biodiesel from Chlorella were comparable to conventional diesel fuel and comply with US standard for Biodiesel. In a word, the approach including
high-density fermentation of Chlorella and enzymatic transesterification process were set up and proved to be a promising alternative for biodiesel production. |
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Keywords: | Agal-fermentation-based microbio-diesel (AFMD) Chlorella protothecoides Fed-batch High-density fermentation Immobilized lipase |
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