Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging |
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| Authors: | Kaspar Podgorski Ewald Terpetschnig Oleksii P Klochko Olena M Obukhova Kurt Haas |
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| Institution: | 1. Department of Cellular and Physiological Sciences and the Brain Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.; 2. SETA BioMedicals, Urbana, Illinois, United States of America.; 3. State Scientific Institution “Institute for Single Crystals” of National Academy of Sciences of Ukraine, Kharkiv, Ukraine.; Instituto de Neurociencias de Alicante UMH-CSIC, Spain, |
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| Abstract: | Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95. |
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