A prevalent alpha-proteobacterium Paracoccus sp. in a population of the Cayenne ticks (Amblyomma cajennense) from Rio de Janeiro,Brazil |
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| Authors: | Erik Machado-Ferreira Joseph Piesman Nordin S. Zeidner Carlos A.G. Soares |
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| Affiliation: | 1.Laboratório de Genética Molecular de Eucariontes e Simbiontes, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.;2.Laboratório de Referência Nacional em Vetores das Riquetsioses, Instituto Oswaldo Cruz-FioCruz, Rio de Janeiro, RJ, Brazil.;3.Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA. |
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| Abstract: | As Rocky Mountain Spotted Fever is the most common tick-borne disease in South America, the presence of Rickettsia sp. in Amblyomma ticks is a possible indication of its endemicity in certain geographic regions. In the present work, bacterial DNA sequences related to Rickettsia amblyommii genes in A. dubitatum ticks, collected in the Brazilian state of Mato Grosso, were discovered. Simultaneously, Paracoccus sp. was detected in aproximately 77% of A. cajennense specimens collected in Rio de Janeiro, Brazil. This is the first report of Paracoccus sp. infection in a specific tick population, and raises the possibility of these bacteria being maintained and/or transmitted by ticks. Whether Paracoccus sp. represents another group of pathogenic Rhodobacteraceae or simply plays a role in A. cajennense physiology, is unknown. The data also demonstrate that the rickettsial 16S rRNA specific primers used forRickettsia spp. screening can also detect Paracoccus alpha-proteobacteria infection in biological samples. Hence, a PCR-RFLP strategy is presented to distinguish between these two groups of bacteria. |
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| Keywords: | Paracoccus Rickettsia amblyommii Amblyomma ixodidae ticks |
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