Characterization of the glyoxalase 1 gene <Emphasis Type="Italic">TcGLX1</Emphasis> in the metal hyperaccumulator plant <Emphasis Type="Italic">Thlaspi caerulescens</Emphasis> |
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Authors: | Marjo Tuomainen Viivi Ahonen Sirpa O Kärenlampi Henk Schat Tanja Paasela Algirdas ?vanys Saara Tuohimetsä Sirpa Peräniemi Arja Tervahauta |
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Institution: | 1.Department of Biosciences,University of Eastern Finland,Kuopio,Finland;2.Genetics, Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam,Amsterdam,The Netherlands;3.Department of Agricultural Sciences,University of Helsinki,Helsingin yliopisto,Finland;4.Vehni?,Finland;5.Coastal Research and Planning Institute,Klaipeda University,Klaipeda,Lithuania |
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Abstract: | Stress tolerance is currently one of the major research topics in plant biology because of the challenges posed by changing
climate and increasing demand to grow crop plants in marginal soils. Increased Zn tolerance and accumulation has been reported
in tobacco expressing the glyoxalase 1-encoding gene from Brassica juncea. Previous studies in our laboratory showed some Zn tolerance-correlated differences in the levels of glyoxalase 1-like protein
among accessions of Zn hyperaccumulator Thlaspi caerulescens. We have now isolated the corresponding gene (named here TcGLX1), including ca. 570 bp of core and proximal promoter region. The predicted protein contains three glyoxalase 1 motifs and several putative
sites for post-translational modification. In silico analysis predicted a number of cis-acting elements related to stress. The expression of TcGLX1 was not responsive to Zn. There was no correlation between the levels of TcGLX1 expression and the degrees of Zn tolerance or accumulation among T. caerulescens accessions nor was there co-segregation of TcGLX1 expression with Zn tolerance or Zn accumulation among F3 lines derived from crosses between plants from accessions with contrasting
phenotypes for these properties. No phenotype was observed in an A. thaliana T-DNA insertion line for the closest A. thaliana homolog of TcGLX1, ATGLX1. These results suggest that glyoxalase 1 or at least the particular isoform studied here is not a major determinant of Zn
tolerance in the Zn hyperaccumulator plant T. caerulescens. In addition, ATGLX1 is not essential for normal Zn tolerance in the non-tolerant, non-accumulator plant A. thaliana. Possible explanations for the apparent discrepancy between this and previous studies are discussed. |
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