Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts. |
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| Authors: | S Huang J D Paulauskis L Kobzik |
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| Affiliation: | Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115. |
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| Abstract: | We have isolated and sequenced overlapping cDNA clones for rat KC*. The 0.93 kb cDNA has a single open reading frame of 288 nucleotides, and substantial sequence identity with the platelet-factor 4 family members mouse KC, hamster gro, and human gro. Using cloned cDNA as a probe, expression of KC mRNA in lavaged rat alveolar macrophages (AMs) increased after lipopolysaccharide (LPS) treatment. We also studied expression in vitro by a rat fetal lung fibroblast cell line, RFL-6. Expression of KC mRNA in RFL-6 cells increased after treatment with interleukin 1 or with conditioned medium from rat AMs treated with LPS. |
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