Electrophysiological properties of frog olfactory supporting cells |
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Authors: | Trotier D |
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Institution: | Neurobiologie Sensorielle, E.P.H.E., Massy, France. trotier@bisance.citi2.fr |
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Abstract: | Cells, identified as supporting cells by Lucifer Yellow injection, were
recorded from slices of frog olfactory epithelium using patch-clamp
recordings. Cell-attached single-channel recordings indicated that the
intracellular potential (IP) was -68 +/- 7 mV (n = 22) with 4 mM K+ in the
bath (K+]o). IP was -67 +/- 4 mV (n = 32) in whole-cell conditions with
100 mM KCl inside the cell, suggesting a low membrane permeability for Cl-.
IP depended on K+]o in a manner described by the Goldman- Hodgkin-Katz
equation with a permeability ratio pk+:PNa+ of 40. The input resistance was
32 +/- 14 M omega (n = 15), indicating a high membrane conductance at rest.
Odorant stimulations evoked passive membrane depolarizations, probably
reflecting an increase in K+]o due to the neuronal activation. Whole-cell
recordings with 100 mM CsCl instead of KCl in the pipette, together with
the block of gap-junctions with octanol, indicated the existence of an
electrical coupling between supporting cells. The electrical coupling
between these glial-like cells could facilitate the clearance of K+ ions
released by olfactory receptor neurons during odorant stimulation.
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