Complex formation between transfer RNAs with complementary anticodons: use of matrix bound tRNA |
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Authors: | H Grosjean C Takada J Petre |
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Institution: | Laboratory of Biological Chemistry, Fac. of Sciences, University of Brussels, Belgium. |
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Abstract: | It is shown that yeast tRNAPhe, chemically coupled by its oxidized 3′CpCpA end behaves exactly as free tRNAPhe in its ability to form a specific complex with tRNA2Glu having a complementary anticodon. The results support models of tRNA in which the 3′CpCpAOH end and the anticodon are not closely associated in the tertiary structure, and provide a convenient tool of general use to characterize others pairs of tRNA having complementary anticodons, as well as for highly selective purification of certain tRNA species. |
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