| 6BA诱导的带正电荷的葡萄叶过氧化物酶 |
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| 引用本文： | 廖祥儒,朱新产,万怡震,贺普超. 6BA诱导的带正电荷的葡萄叶过氧化物酶[J]. 植物生理与分子生物学学报, 1999, 0(1) |
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| 作者姓名： | 廖祥儒 朱新产 万怡震 贺普超 |
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| 作者单位： | 河北大学生物技术中心!河北保定071002(廖祥儒)，西北农业大学园艺系!陕西杨陵712100(朱新产，万怡震，贺普超) |
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| 摘 要： | 河岸葡萄叶的带正电荷过氧化物酶（ｃａｔｉｏｎｉｃ ｐｅｒｏｘｉｄａｓｅ， ＣＰＯＤ） 可受６ＢＡ 诱导，但盐、Ｈ２Ｏ２ 或Ｆｅ２＋＋ Ｈ２Ｏ２ 均使叶片ＣＰＯＤ 活性明显下降，而高浓度的无机盐明显刺激纯ＣＰＯＤ 活性增加。在以愈创木酚为底物时ＣＰＯＤ 最适ｐＨ 为４ ．６０ ～５ ．７５ ，对Ｈ２Ｏ２ 的表观Ｖｍａｘ 和Ｋｍ 值分别为１１０ Ｕ／ｍｇ 蛋白和１ ．１５ｍ ｍｏｌ／Ｌ。
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| 关 键 词： | 带正电荷的过氧化物酶 6BA 无机盐 葡萄 |
A 6 Benzylaminopurine Induced Cationic Peroxidase in Grape Leaves |
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| LIAO Xiang Ru ,ZHU Xin Chan ,WAN Yi Zhen and HE Pu Chao. A 6 Benzylaminopurine Induced Cationic Peroxidase in Grape Leaves[J]. Journal Of Plant Physiology and Molecular Biology, 1999, 0(1) |
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| Authors: | LIAO Xiang Ru ZHU Xin Chan WAN Yi Zhen HE Pu Chao |
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| Affiliation: | LIAO Xiang Ru 1,ZHU Xin Chan 2,WAN Yi Zhen 2 and HE Pu Chao 2 |
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| Abstract: | A cationic peroxidase (CPOD) was purified from leaves of Vitis vulpina L, and some of its characteristics were analyzed. The results showed that the cationic peroxidase could be induced by 6 benzylaminopurine. It also inhibited markedly by salinity, H 2O 2 or Fe 2 H 2O 2, in vivo . But the purified CPOD was stimulated significantly by high concentrations of inorganic salts. The optimum pH of the CPOD activity was between 4.60 and 5.75 with guaiacol as substract. The apparent V max and K m were 110 U/mg protein and H 2O 2 1.15 mmol/L, respectively. |
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| Keywords: | cationic peroxidase 6 benylaminopurine inorganic salt Vitis vulpina |
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