| 高效液相色谱法测定粮食作物中的色氨酸 |
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| 引用本文： | 刘惠文.高效液相色谱法测定粮食作物中的色氨酸[J].氨基酸和生物资源,1997,19(4):29-31. |
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| 作者姓名： | 刘惠文 |
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| 作者单位： | 中国林业科学研究院分析中心 |
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| 摘 要： | 用ＨＰＬＣ法测定粮食作物中的色氨酸，实验采用阳离子交换柱，以０．１ｍｏｌ／ＬＣＨ３ＣＯＯＮＨ４为流动相，荧光检测器中ｅｘ２８０ｎｍ，ｅｍ３４０ｎｍ检测，色氨酸和酪氨酸分离很好，回收率在９５％以上。
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| 关 键 词： | 色氨酸 阳离子交换柱 高效液相色谱 流动相 荧光检测器 |
Determination of the Tryptophan in Cereal Crops with HPLC |
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| Liu Huiwen.Determination of the Tryptophan in Cereal Crops with HPLC[J].Amino Acids & Biotic Resources,1997,19(4):29-31. |
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| Authors: | Liu Huiwen |
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| Institution: | ,Liu Huiwen (The Research Institute of Forest Environmental Science, The Chinese Academy of Forestry, Beijing 10091) |
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| Abstract: | The tryptophan in alkaline hydrolysate of cereal crops was determined with HPLC using Cation-exchange column and 0.1 mol/L CH3COONH4 as mobile phase and fluorescence detector at ex:280nm,em:340nm. Trp and Tyr were seperated well.The recovery rate of the method was over 95%。 |
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| Keywords: | Tryptophan cation -exchange column The HPLC fluorescence detector |
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