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Amelioration of chilling stress by triadimefon in cucumber seedlings
Authors:Zhaozhong Feng  Anhong Guo  Zongwei Feng
Institution:(1) Research Center for Eco-environmental Sciences, Department of System Ecology, The Chinese Academy of Science, Beijing, 100085, China;(2) Chinese Academy of Meteorological Science, Beijing, 100081, China
Abstract:Cucumber (Cucumis satvus L.) seeds were imbibed in distilled water (control) and 10 mg l–1 triadimefon (TDM) for 10 h and then grown in a plant growth chamber with a light/dark temperature of 28/20 °C and a photoperiod of 14 h with a light intensity of 60 µmol m–2 s–1. 14-day-old seedlings were exposed to chilling stress with a light/dark temperature of 6/3 °C for 4 d. TDM improved the growth rate of cucumber seedling subjected to chilling stress and increased photosynthetic pigments contents and relative water content compared with the control at the end of chilling stress. Chilling stress decreased protein content and the activities of SOD, CAT and POD, but it increased proline, H2O2 and MDA accumulation, and relative electrical conductivity. TDM ameliorated the injury caused by chilling stress by preventing decreases in protein content and the activities of SOD, CAT and POD and by inhibiting increases in proline, H2O2 and MDA contents, and relative electrical conductivity, which suggested that TDM ameliorated the negative effect of chilling stress.
Keywords:Antioxidant enzymes  Chemical composition  Cucumis satvus  Lipid peroxidation  Reactive oxygen species
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