Analysis of thiamine and its phosphate esters by high-performance liquid chromatography. |
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| Authors: | K Ishii K Sarai H Sanemori T Kawasaki |
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| Affiliation: | 1. Department of Neuropsychiatry, Hirashima University School of Medicine, Hiroshima 734, Japan;2. Department of Biochemistry, Hirashima University School of Medicine, Hiroshima 734, Japan |
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| Abstract: | High-performance liquid chromatography was used to separate thiamine and its phosphate esters after conversion to corresponding highly fluorescent thiochrome derivatives by alkaline oxidation. These compounds were absorbed on LiChrosorb-NH2, eluted with acetonitrile-90 mm potassium phosphate buffer (pH 8.4), and determined spectrofluorometrically. A complete, rapid, and quantitative separation of thiochrome and its phosphate derivatives was made and the minimum amount detected was 1 pmol for each of these compounds. |
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| Keywords: | To whom all correspondence should be addressed. |
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