| 单克隆抗体与抗血清联合应用建立血清CMLC测定方法的研究 |
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| 引用本文： | 霍勇,汪丽蕙,刘新军,杨非易,杜国光,葛韵琴.单克隆抗体与抗血清联合应用建立血清CMLC测定方法的研究[J].中国生物化学与分子生物学报,1993,9(4):454-459. |
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| 作者姓名： | 霍勇 汪丽蕙 刘新军 杨非易 杜国光 葛韵琴 |
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| 作者单位： | 北京医科大学第一医院心内科，北京医科大学第一医院心内科，北京医科大学生物化学教研室，北京医科大学生物化学教研室，北京医科大学生物化学教研室，北京医科大学生物化学教研室 北京 100034，北京 100034，北京 100083，北京 100083，北京 100083，北京 100083 |
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| 摘 要： | 单克隆抗体(McAb)和抗血清各有特点。本文提纯人心肌肌球蛋白轻链(CMLC)并制备其单克隆抗体和抗CMLC兔血清(下称抗血清),试建立测定血清CMLC的酶联免疫(ELISA)方法。通过比较,McAb和抗血清联合应用可提高测定方法的灵敏度和特异性;并对各反应试剂的工作浓度进行确定,建立了McAb(1C_(11)-D_7株)为铺底抗体,抗血清为覆盖抗体,碱性磷酸酶标记的羊抗兔IgG为第三抗体的三抗体酶联免疫夹心测定血清CMLC的方法(MP-ELISA)。血清CMLC最小可测浓度为2.5ng/mL。
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| 关 键 词： | McAb 抗血清 CMLC ELISA |
| 收稿时间： | 1993-08-20 |
The Combination Usage Monoclonal Antibody and Antiserum in Determination of Serum Cardiac Myosin Light Chains |
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| Ho,Yong Wang,Li-hui Liu,Xin-jun Yang,Fei-yi Du,Guo-guang Ge,Yun-qin.The Combination Usage Monoclonal Antibody and Antiserum in Determination of Serum Cardiac Myosin Light Chains[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(4):454-459. |
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| Authors: | Ho Yong Wang Li-hui Liu Xin-jun Yang Fei-yi Du Guo-guang Ge Yun-qin |
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| Institution: | (The First School of Clinical Medicine, 100034 ~* The School of Basic Medical Science, 100083 Beijing Medical University |
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| Abstract: | An enzyme-linked immunosorbent assay (ELISA) for serum cardiac myosin light chains (CMLC), employing both monoclonal antibody (McAb) and antiserum, has been developed. After the different pairs of McAb and antiserum were compared and optimal antibodies and their working dilutions were determined, the protocol of the MP-ELISA was finally set: McAb 1C11-D7 (30 μg/mL) as the coating antibody, antiserum (1: 200) as the successive antibody and goat-anti-rabbit IgC labeled with alkaline phoaphatase (1: 800) as the third antibody. With the combination of McAb and antiserum, the assay demonstrated high specificity and sensitivity with minimum measurable concentration of 2.5ng/mL and enjoyed important clinical feasibility. |
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| Keywords: | Monoclonal antibody Antiserum Cardiac myosin light chains Enzyme-linked immunosorbent assay |
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