Effects of phosphorus supply on in vitro growth and phosphatase activity of Frankia isolates from Casuarina |
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Authors: | Yang Y Shipton WA Reddell P |
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Institution: | (1) Department of Microbiology and Immunology, James Cook University, Townsville, QLD, 4811, Australia;(2) CSIRO, Davies Laboratory, PO Aitkenvale, Townsville, QLD, 4814, Australia |
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Abstract: | An in vitro experiment was conducted to investigate the effects of different sources and levels of P supply on growth, viability and phosphatase activity of three tropical Frankia strains isolated from Casuarina. P concentration for optimum growth was between 0.1 and 10.0 M in the absence of external combined nitrogen. Specific viability was not influenced by P supply. Morphological features of Frankia, such as hyphal length and vesicle numbers, were found to largely mirror growth. Phosphatase activity was detected in all three Frankia strains and was highest when P was omitted from the culture solution. There were more than 10-fold differences between the Frankia strains in the level of phosphatase activities shown. This study suggested that soils low in P are unlikely to restrict micro-symbiont growth activity. |
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Keywords: | Frankia phosphatase activity phosphorus protein viability |
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