Carotene production from waste cooking oil by Blakeslea trispora in a bubble column reactor: The role of oxidative stress |
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Authors: | Konstantina Nanou Triantafyllos Roukas Emmanuel Papadakis Parthena Kotzekidou |
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Institution: | 1. Laboratory of Food Engineering and Processing, Department of Food Science and Technology, Aristotle University, Thessaloniki, Greece;2. Pesticide Science Laboratory, Aristotle University, Thessaloniki, Greece;3. Laboratory of Food Microbiology and Hygiene, Department of Food Science and Technology, Aristotle University, Thessaloniki, Greece |
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Abstract: | The oxidative stress induced by hydroperoxides and reactive oxygen species (ROS) during carotene production from waste cooking oil (WCO) and corn steep liquor (CSL) by the fungus Blakeslea trispora in a bubble column reactor was investigated. The specific activities of the intracellular enzymes superoxide dismutase (SOD) and catalase (CAT) as well as the micromorphology of the fungus were measured in order to study the response of the fungus to oxidative stress. The changes of the morphology of microorganism leaded to pellets formation and documented using a computerized image analysis system. As a consequence of the mild oxidative stress induced by hydroperoxides of WCO and ROS a significant increase in carotene production was obtained. The highest carotene concentration (980.0 mg/l or 51.5 mg/g dry biomass) was achieved in a medium consisted of CSL (80.0 g/L) and WCO (50.0 g/L) at an aeration rate of 5 vvm after 6 days of fermentation. In this case the carotenes produced consisted of β‐carotene (71%), γ‐carotene (26%), and lycopene (3%). The strong oxidative stress in the fungus caused a significant increase of γ‐carotene concentration. Bubble column reactor is a useful fermentation system for carotene production in industrial scale. |
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Keywords: | Blakeslea trispora Bubble column reactor Carotenes Oxidative stress Waste cooking oil |
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