Novel approach for the prediction of cell densities and viability in standardized translucent cell culture biochips with near infrared spectroscopy |
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| Authors: | Marko Gröger Matthias Lange Knut Rennert Tobias Kaschowitz Holger Plettenberg Martin Hoffmann Alexander S. Mosig |
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| Affiliation: | 1. Center for Sepsis Control and Care, Jena University Hospital, Jena, Germany;2. Institute of Biochemistry II, Jena University Hospital, Jena, Germany;3. fzmb Research Centre of Medical Technology and Biotechnology, Bad Langensalza, Germany;4. Arthrospec GmbH, Erfurt, Germany |
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| Abstract: | Near infrared spectroscopy is a rapid and nondestructive method for compositional analysis of biological material. The technology is widely used within bioreactors and possesses potential as a standardized method for quality control in miniaturized microfluidic cell culture systems. Here, we established a method for quantification of cell density and viability of adherent HepaRG cells cultured in a translucent, miniaturized cell culture biochip. The newly developed statistical models for interpretation of near infrared spectroscopy from biochips are the basis for a novel method of fast, continuous, and contact‐free analysis of cell viability and real‐time monitoring of cell growth. The technique thus paves the way for a robust and reliable high‐throughput analysis of biochip‐embedded cell cultures. |
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| Keywords: | Biochips Cell culture HepaRG Near infrared spectroscopy Partial least squares |
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