Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola |
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Authors: | Rustern I Aminov Takafumi Nagamine Koretsugu Ogata Mutsumi Sugiura Kiyoshi Tajima Yoshimi Benno |
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Institution: | STAFF-Institute, Ippaizuka 446-1, Kamiyokoba, Tsukuba, Ibaraki 305, Japan; JCM, RIKEN, Wako 351-01, Japan |
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Abstract: | Abstract Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recE from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa. P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coli recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen. |
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Keywords: | RecA Prevotella ruminicola Escherichia coli Complementation |
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