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Influence of Insect Larvae and Plant Roots on the Host-finding Behaviour of Heterorhabditis megidis
Authors:M I C Boff  G L Wiegers  P H Smits
Abstract:We studied the host-finding and dispersion behaviour of Heterorhabditis megidis (strain NLHE 87.3) in the presence of Galleria mellonella or Otiorhynchus sulcatus larvae and strawberry roots. In large Petri dishes (19 cm diameter) filled with moist sand (8% w/w), and incubated at 15°C over 24 h, infective juveniles (IJs) responded positively to the presence of G. mellonella , to roots of a single strawberry plant and to O. sulcatus larvae in direct contact with roots of a single strawberry plant. A neutral or negative response was observed when IJs were presented with only O. sulcatus larvae or a combination of several strawberry plants with O. sulcatus larvae, either in contact or not in contact with the roots. IJs responded strongly to the combination of plant roots and feeding larvae indicating that the tritrophic interaction formed by IJs - O. sulcatus larvae - strawberry plants may be an infochemical-mediated interaction.
Keywords:Entomopathogenic  Nematodes  Nematode  Behaviour  Plant  Roots  Heterorhabditis  Megidis  Galleria  Mellonella  Otiorhynchus  Sulcatus  Strawberry
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