Optimizing parameters for correlative immunogold localization by video-enhanced light microscopy, high-voltage transmission electron microscopy, and field emission scanning electron microscopy |
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| Authors: | S R Simmons J B Pawley R M Albrecht |
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| Institution: | Department of Veterinary Science, University of Wisconsin, Madison 53706. |
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| Abstract: | Correlative video-enhanced light microscopy, high-voltage transmission electron microscopy, and low-voltage high resolution scanning electron microscopy were used to examine the binding of colloidal gold-labeled fibrinogen to platelet surfaces. Optimal conditions for the detection of large (18 nm) and small (3 nm) gold particles are described. |
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