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Evaluation of the genetic variability of 23 bovine microsatellite markers in four Belgian cattle breeds
Authors:L J Peelman  F Mortiaux  A Van Zeveren  A Dansercoer  G Mommens  F Coopman  Y Bouquet  A Burny  R Renaville  & D Portetelle
Institution:Research Centre for Molecular &Biochemical Genetics in Domestic Animals, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke, Belgium,;Department of Applied Biochemistry and Biology, Faculty of Agronomy, B 5030 Gembloux, Belgium,;Cattle Blood Typing Laboratory, National Cattle Breeders Association, Malle, Belgium
Abstract:The polymorphism of 23 microsatellites in the four main cattle breeds in Belgium (Holstein Friesian, Belgian Blue, Belgian Red Pied and East Flemish) was analysed. Heterozygosity, polymorphism information content, the effective number of alleles, exclusion probability and the probability of genotypic identity for two random individuals were calculated for all microsatellites and all breeds. The Belgian Blue breed is generally a little less polymorphic in comparison with the other three breeds. Estimates of the genetic distances between these breeds confirmed the widely accepted proposition that the Belgian Blue is the most genetically distinct of these breeds. The three other breeds are likely to become one population, given current breeding strategies. Exclusion probabilities in parentage control cases are >0·9999 in all four breeds when all 23 microsatellites are used and >0·98 with only the two most polymorphic multiplexes.
Keywords:cattle  microsatellite  genetic distance  variability
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