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Protein phosphorylation in human peripheral lymphocytes - stimulation by phytohemagglutinin and N6 monobutyryl cyclic AMP. |
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| Authors: | H J Wedner C W Parker | |
| Affiliation: | Washington Univ. School of Medicine, Dept. of Medicine, St. Louis, Mo. USA | |
| Abstract: | Protein phosphorylation was studied in human peripheral lymphocytes. The cells were preincubated with ![/></figure>, exposed to phytohemagglutinin (PHA), N<sup>6</sup> monobutyryl cAMP (MBcAMP) or 8 bromo cGMP (BcGMP), homogenized and analyzed by SDS-polyacrylamide gel electrophoresis. Both PHA and MBcAMP produced early increases in the [<sup>32</sup>P]content of multiple proteins in the 30,000–100,000 molecular weight range. After further incubation with PHA there was a shift in the phosphorylation response to smaller molecular weight proteins. BcGMP (1 mM-10 pM) had no effect on protein phosphorylation. These results suggest a role for cAMP in the early action of PHA on human peripheral lymphocytes.</td>
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