An operative approach to correct CD spectra distortions due to absorption flattening |
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Authors: | Castiglioni Ettore Lebon France Longhi Giovanna Gangemi Roberto Abbate Sergio |
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Affiliation: | JASCO Corporation, Hachioji-Shi, Tokyo, Japan. ettore.castiglioni@jasco-europe.com |
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Abstract: | We discuss the influence of absorption flattening (AF) on CD spectra, aiming at finding a simple way to compensate the induced distortions. A simple algebraic formalism is proposed based on an unconventional measuring approach, which makes use of a commercial unit. Validation of the proposed compensation method for AF is conducted not only on the already studied CD Vis spectral region of a Co chiral complex with the use of an AF emulator, but also on the far UV CD spectra of poly-L-glutamic acid suspensions with different turbidity levels. Comparison with some correction factors proposed in the literature is presented. |
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