Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins |
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Authors: | Miyamoto Kentaro Sakurai Hidetaka Sugiura Takeyuki |
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Institution: | Discovery Research Laboratory, Tokyo R&D Center, Daiichi Pharmaceutical Co., Ltd., Daiichi-Sankyo group, Tokyo, Japan. |
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Abstract: | RNF43 is an oncogenic RING finger protein overexpressed in colorectal cancer. To dissect its biological functions, we explored RNF43-interacting proteins by pull-down assay and MS. We identified a heterodimer, p54nrb and PSF, as RNF43's binding partners and confirmed their physical interaction in vivo by the co-immunoprecipitation experiment. Immunofluorescence analysis revealed that co-expression of PSF relocates RNF43 from the nuclear periphery to the nucleoplasm. Thus, proteomic identification of RNF43-associated proteins sheds light on its dynamic interaction network in nuclear events. |
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Keywords: | MS p54nrb PSF RING finger domain RNF43 |
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