Evaluation ofl-lactic acid production in batch, fed-batch, and continuous cultures ofRhizopus sp. MK-96-1196 using an airlift bioreactor |
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Authors: | Tiejun Liu Shigenobu Miura Tomohiro Arimura Min-Yi Tei Enoch Y. Park Mitsuyasu Okabe |
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Affiliation: | (1) The United Graduate School of Agricultural Science of Gifu University, 1-1, Yanagido, 501-1193 Gifu, Japan;(2) Tokyo Laboratories, Musashino Chemical Laboratory Ltd., 16-2, Miyamae 1-Chome, 168-0081 Suginami-ku, Japan;(3) Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529 Shizuoka, Japan |
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Abstract: | Various processes which producel-lactic acid using ammonia-tolerant mutant strain,Rhizopus sp. MK-96-1196, in a 3 L airlift bioreactor were evaluated. When the fed-batch culture was carried out by keeping the glucose concentration at 30 g/l, more than 140 g/l ofl-lactic acid was produced with a product yield of 83%. In the case of the batch culture with 200 g/l of initial glucose concentration, 121 g/L ofl-lactic acid was obtained but the low product yield based on the amount of glucose consumed. In the case of a continuous culture, 1.5 g/l/h of the volumetric productivity with a product yield of 71% was achieved at dilution rate of 0.024 h−1. Basis on these results three processes were evaluated by simple variable cost estimation including carbon source, steam, and waste treatment costs. The total variable costs of the fed-batch and continuous cultures were 88% and 140%, respectively, compared to that of batch culture. The fed-batch culture with highl-lactic acid concentration and high product yield decreased variable costs, and was the best-suited for the industrial production ofl-lactic acid. |
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Keywords: | Rhizopus font-variant:small-caps" >l-lactic acid airlift bioreactor ammonia-tolerant strain fed-batch culture |
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