Characterisation of the extracellular polysaccharides produced by isolates of the fungus Acremonium |
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| Authors: | Schmid Frank Separovic Frances McDougall Barbara M Stone Bruce A Brownlee Robert T C Seviour Robert J |
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| Institution: | Biotechnology Research Centre, La Trobe University, Bendigo, VIC 3550, Australia. |
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| Abstract: | Solid state (13)C NMR studies of the extracellular glucans from the fungi Acremonium persicinum C38 (QM107a) and Acremonium sp. strain C106 indicated a backbone of (1-->3)-beta-linked glucosyl residues with single (1-->6)-beta-linked glucosyl side branches for both glucans. Analyses of enzymatic digestion products suggested that the average branching frequency for the A. persicinum glucan (66.7% branched) was much higher than that of the Acremonium sp. strain C106 glucan (28.6% branched). The solid state (13)C NMR spectra also indicated that both glucans are amorphous polymers with no crystalline regions, and the individual chains are probably arranged as triple helices. |
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| Keywords: | Acremonium (1→3 1→6)-β-glucans Solid state 13C NMR Fungal extracellular polysaccharide Enzymatic hydrolysis |
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