Association of Cbl with Fms and p85 in response to macrophage colony-stimulating factor |
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Authors: | Ota J Sato K Kimura F Wakimoto N Nakamura Y Nagata N Suzu S Yamada M Shimamura S Motoyoshi K |
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Affiliation: | Biochemical Research Laboratory, Morinaga Milk Industry, Higashihara 5-1-83, Zama, Kanagawa, Japan. |
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Abstract: | Tyrosine phosphorylation of Cbl and its association with signal-transducing molecules in response to macrophage colony-stimulating factor (M-CSF) were analyzed by using cell lines which express the wild-type and a mutant M-CSF receptor, Fms. We found that in a clone, F723 TF-1 cells expressing mutant Fms in which tyrosine 723 had been substituted with phenylalanine, the M-CSF stimulation-dependent association between Cbl and Fms was markedly impaired. However, phosphorylation of Cbl and its association with the p85 subunit of phosphatidylinositol 3-kinase were induced in these mutant cells as seen in the wild-type fms transfectant. These results suggest that phosphorylation of tyrosine 723 is particularly important for the recruitment of Cbl to the M-CSF receptor, but is not required for the phosphorylation and binding of Cbl to signal-transducing molecules such as p85. |
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