小单孢菌叠加诱变与发酵过程的研究

采用庆大霉素产生菌—绛红小单孢菌 (M. purpurea) 在培养36h时添加15μg/ml的青霉素,对培养至48h对数生长期的菌体进行紫外线照射+亚硝基胍复合处理,置培养箱中37℃培养5~8d待长出菌落后再用紫外线照射叠加处理,获得一高产菌株N-14。试管发酵生测效价1913u/ml(对照625u/ml);摇瓶发酵培养120h达2178(u/ml),比对照(1243u/ml)提高75.2%;并进行了5L发酵罐发酵培养,发酵过程中测定了发酵液还原糖、总糖、氨基氮、pH、菌体浓度等代谢参数,探索了适合N-14的培养条件,连续5批次生测效价平均1967u/ml,较对照1171u/ml提高了68%, 效果明显。

Study on Superimposed Mutation on Micromonospora purpurea and Fermentation Process in 5L Fermentor

Gentamicin producing strain Micromonospora purpurea was treated with 15μg/ml penicillin when M. purpurea had cultured 36h, and the cells that had cultured for 48h at logarithmic growth phase were treated with UV and NTG, then they were cultured in incubator at 37℃ for 5~8 days, and when the colonies grew out, the cells were treated by UV again. As a result, a high yield strain N-14 was obtained. Compared to control, the biological titer of N-14 fermented in tube was increased from 625u/ml to 1913u/ml, and the biological titer of N-14 fermented in flask was 2178u/ml and increased by 75.2% compared to control 1243(u/ml). Besides, the reducing sugar, total sugar, amino nitrogen, pH, cell concentration and other metabolic parameters were determined during fermentation process in 5L fermentor, and the culture condition was researched. The average biological titer of 5 batches was 1967u/ml and was increased by 68% compared to control 1171u/ml.