The interaction of some microbial and mammalian proteinases with human blood trypsin inhibitors |
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| Authors: | J Tuma L Parker |
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| Affiliation: | 1. Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada;2. Quantum and Nanotechnologies Research Centre, National Research Council Canada, 11421 Saskatchewan Dr NW, Edmonton, AB T6G 2M9, Canada;3. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB T6G 2G2, Canada;4. Aquatic and Crop Resource Development Research Centre, National Research Council Canada, 110 Gymnasium Pl, Saskatoon, SK S7N 0W9, Canada;5. Department of Chemical and Biological Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK S7N 5A9, Canada;1. Science and Technology Center for Sustainability (CCTS), Federal University of São Carlos (UFSCar), João Leme dos Santos, km 110, 18052-780 Sorocaba, Brazil;2. Institute of Science and Technology (ICT), Federal University of São Paulo (UNIFESP), 12231-280 São José do Campos, SP, Brazil;3. Portable Chemical Sensors Lab, Department of Analytical Chemistry, Institute of Chemistry, State University of Campinas (UNICAMP), P.O. Box 6154, 13083-970 Campinas, SP, Brazil;4. Polymer Materials Characterization Laboratory (LCaMP), Technological College of Sorocaba (FATEC), Eng. Carlos Reinaldo Mendes, 2015, 18013-280 Sorocaba, SP, Brazil;5. Science and Technology Institute of Sorocaba (ICTS), São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180 Sorocaba, Brazil;6. Department of Physics, Paulista State University (UNESP), 19060-900 Presidente Prudente, SP, Brazil;7. Universidade Estadual de Campinas, Instituto de Física Gleb Wataghin, Campinas, SP 13083-859, Brazil |
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| Abstract: | It has been shown that trypsin inhibitors in human serum inhibit the microbial serine proteinases subtilisin, aspergillopeptidase B, and the enzymes from Streptomyces griseus. The inhibition of these enzymes by serum was compared with the inhibition of the homologous mammalian proteinases trypsin, chymotrypsin, and elastase. The microbial proteinases were found to interact with larger quantities of the inhibitors in serum and are, therefore, less effectively inhibited by serum than the mammalian enzymes.In addition to serine proteinases, the bacterial metalloenzyme thermolysin also interacts with the inhibitors but is not appreciably inhibited by them.It was shown that the enzyme molecules must have intact active sites for the enzyme-inhibitor reaction to occur.It is suggested that the function of trypsin inhibitors is to protect tissues against the action of serine proteinases of both endogenous and exogenous origin. |
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