Low-temperature induced transmembrane potential changes in the liverwort Conocephalum conicum |
| |
Authors: | Krol Elzbieta Dziubinska Halina Trebacz Kazimierz |
| |
Affiliation: | Department of Biophysics, Institute of Biology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland. krolik@biotop.umcs.lublin.pl |
| |
Abstract: | Intracellular microelectrode measurements revealed that the liverwort Conocephalum conicum generates all-or-none action potentials (APs) in response to a sudden temperature drop. In plants with anion and potassium conductance blocked, dose-dependent voltage transients (VTs) were evoked by cold stimuli. These VTs did not propagate. When the external concentration of Ca(2+) was decreased or calcium channel inhibitors (La(3+), Gd(3+), verapamil, Mg(2+), Mn(2+)) were used, inhibition of VTs was observed. Amplitudes of both APs and VTs grew when Sr(2+) ions, known to release calcium from internal stores, were added to the medium. Neomycin, which suppresses phospholipase C and indirectly affects inositol triphosphate formation, caused substantial inhibition of both APs and VTs. It is concluded that a temperature drop elucidated membrane potential changes due to calcium influx both from external and internal stores. |
| |
Keywords: | |
本文献已被 PubMed Oxford 等数据库收录! |
|