Glycoproteins of drusen and drusen-like lesions |
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Authors: | Yvonne D’souza Carolyn J P Jones Richard Bonshek |
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Institution: | (1) Academic Unit, Manchester Royal Eye Hospital, Oxford Road, Manchester, M13 9WH, UK;(2) Maternal and Fetal Health Research Centre, University of Manchester, St Mary’s Hopsital, Hathersage Road, Manchester, M13 0JH, UK |
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Abstract: | Drusen are a marker of age-related macular degeneration (AMD). Lesions similar to drusen, both in histology and their clinical
appearance, are also seen in choroidal tumours, chronic inflammatory and degenerative conditions of the eye, and in mesangiocapillary
glomerulonephritis type II (MCGN-II). This study aims to compare the saccharide composition of these drusen-like lesions in
the various ocular pathological groups and in MCGN-II. Formalin fixed and paraffin wax embedded tissue from 21 eyes was studied.
The histological diagnoses included AMD, retinal detachment, phthisis bulbi following failed retinal detachment surgery, malignant
melanoma, long-standing uveitis, glaucoma and MCGN II. Glycosylation was examined using a panel of twenty biotinylated lectins
and an avidin-peroxidase DAB-cobalt revealing system, with and without neuraminidase pre-treatment. High mannose, bi/tri-nonbisected
and bisected complex N-glycan, N-acetyl glucosaminyl, galactosyl and sialyl residues were found to be expressed by drusen,
while treatment with neuraminidase exposed subterminal N-acetyl galactosamine and galactosyl residues. Similar binding patterns
were found in the various pathological groups studied. As there was no significant difference in the lectin-binding pattern
in drusen in different pathologies, a common pathogenesis or at least a final common pathway for the elaboration of carbohydrate
components of drusen is suggested. |
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Keywords: | Drusen Lectins Eye Age-related macular degeneration Glycoproteins Pathology |
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