Production of succinate and polyhydroxyalkanoate from substrate mixture by metabolically engineered Escherichia coli |
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Authors: | Kang Zhen Du Lili Kang Junhua Wang Yang Wang Qian Liang Quanfeng Qi Qingsheng |
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Affiliation: | a State Key Laboratory of Microbial Technology, Life Science School, Shandong University, Jinan 250100, PR China b National Glycoengineering Research Center, Life Science School, Shandong University, Jinan 250100, PR China |
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Abstract: | The strategic design of this study aimed at producing succinate and polyhydroxyalkanoate (PHA) from substrate mixture of glycerol/glucose and fatty acid in Escherichia coli. To accomplish this, an E. coli KNSP1 strain derived from E. coli LR1110 was constructed by deletions of ptsG, sdhA and pta genes and overexpression of phaC1 from Pseudomonas aeruginosa. Cultivation of E. coli KNSP1 showed that this strain was able to produce 21.07 g/L succinate and 0.54 g/L PHA (5.62 wt.% of cell dry weight) from glycerol and fatty acid mixture. The generated PHA composed of 58.7 mol% 3-hydroxyoctanoate (3HO) and 41.3 mol% 3-hydroxydecanoate (3HD). This strain would be useful for complete utilization of byproducts glycerol and fatty acid of biodiesel production process. |
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Keywords: | Succinate PHA Escherichia coli Metabolic engineering Biorefinery |
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