Molecular characteristics of the <Emphasis Type="Italic">SLA-2</Emphasis> gene from Chinese Hebao pigs |
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Authors: | F -Sh Gao J Bai X -H Zhang W -J Zhang D Guo Sh Zhang |
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Institution: | 1.Department of Biochemistry and Molecular Biology, College of Bioengineering,Dalian University,Dalian, Liaoning,China;2.Institute of Animal Husbandry of Liaoning Province,Liaoyang, Liaoning,China |
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Abstract: | To study the molecular characteristics of swine leukocyte antigen (SLA) class I from the Hebao pig, a rare inbreed in China,
a pair of primers was designed to amplify the SLA-2 gene (SLA-2-HB) and then the molecular characteristics of the gene were analyzed by computer. After cloning, sequencing and computer analysis,
four SLA-2-HB alleles were found, all of 1119 bp. Sites 3–1097 were an open reading frame encoding 364 amino acids with two sets of intra-chain
disulfide bonds comprising four cysteines situated in sites 125, 188, 227 and 283. By alignment of SLA-2-HB sequences with other SLA-2 alleles in the DNA Data Bank of Japan/European Molecular Biology Laboratory/GenBank database, nine key variable amino acid
sites were found in the extracellular domain of the SLA-2-HB alleles at sites 23(F), 24(I), 43(A), 44(K), 50(Q), 73(N), 95(I), 114(R) and 216(S), which could be used to differentiate
other SLA-2 alleles. The amino acid identities between SLA-2-HB and other SLA-2, SLA-3 and SLA-1 alleles were 87.1–97.0%, 85.0–93.9% and 83.3–88.6%, respectively. The phylogenetic tree of SLA-2-HB showed that it was relatively independent of the other SLA-2 genes. Furthermore, the SLA-2-HB alleles were similar to HLA-B15 and HLA-A2 functional domains and preserved some functional sites of HLA-A2. It was concluded that SLA-2-HB is an allele of SLA-2 and that the Hebao pig might have evolved independently in China. |
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