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Detection and identification by PCR of a highly virulent phylogenetic subgroup among extraintestinal pathogenic Escherichia coli B2 strains
Authors:Bidet Philippe  Metais Arnaud  Mahjoub-Messai Farah  Durand Lionel  Dehem Marie  Aujard Yannick  Bingen Edouard  Nassif Xavier  Bonacorsi Stéphane
Affiliation:Service de Microbiologie, H?pital Robert Debré, 48 Bd Sérurier, 75395 Paris cedex 19, France. edouard.bingen@rdb.ap-hop-paris.fr.
Abstract:Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches.
Keywords:
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