Detection and identification by PCR of a highly virulent phylogenetic subgroup among extraintestinal pathogenic Escherichia coli B2 strains |
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Authors: | Bidet Philippe Metais Arnaud Mahjoub-Messai Farah Durand Lionel Dehem Marie Aujard Yannick Bingen Edouard Nassif Xavier Bonacorsi Stéphane |
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Affiliation: | Service de Microbiologie, H?pital Robert Debré, 48 Bd Sérurier, 75395 Paris cedex 19, France. edouard.bingen@rdb.ap-hop-paris.fr. |
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Abstract: | Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches. |
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