Structure of the K95 antigen from Escherichia coli O75:K95:H5, a capsular polysaccharide containing furanosidic KDO-residues |
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| Authors: | T Dengler B Jann K Jann |
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| Affiliation: | 1. Pathobiology Group, Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine and Zootechnics, Universidad Nacional de Colombia (UN), Bogotá D.C., Colombia;2. Laboratory of Veterinary Pathology, Universidad de Ciencias Aplicadas y Ambientales, Bogotá D.C., Colombia;1. Basic Medical College, Shanxi Medical University, Taiyuan 030001, China;2. School of Chemistry, Biology and Environmental Engineering, Xiangnan University, Chenzhou 423000, China;3. College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China |
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| Abstract: | The structure of the K95 antigenic capsular polysaccharide (K95 antigen) of Escherichia coli O75:K95:H5 was elucidated by determination of the composition, 1H- and 13C-n.m.r. spectroscopy, periodate oxidation, and methylation analysis. The K95 polysaccharide, which contains furanosidic 3-deoxy-D-manno-2-octulosonic acid (KDOf) residues, consists of----3)-beta-D-Rib-(1----8)-KDOf-(2----repeating units, has a molecular weight of approximately 25,000 (approximately 65 repeating units), and is randomly O-acetylated (1 acetyl group per repeating unit at unknown positions). |
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