Synthesis and Characterization of Nicotiana-Solanum Graft Chimeras

Intergeneric Nicotiana tabacum L./Solanum laciniatum Ait. graftchimeras were produced from decapitated grafts made betweenthese two graft compatible species. Graft unions were treatedwith or without either the auxin, p-chlorophenoxyacetic acid(p-CPA) or the cytokinin, benzylaminopurine (BAP). While BAPwas inhibitory to shoot formation, p-CPA increased the numberof adventitious shoots and raised the frequencies of shoot-forminggrafts obtained. Approximately 9% (14/151) of the shoots producedat p-CPA-treated graft unions were intergeneric chimeras. Theauxin application significantly increased levels of chimeralshoot recovery numbers thus indicating a direct auxin effecton chimeral shoot production. The types and numbers of chimerasproduced were independent of the scion/stock graft combinationemployed. All chimeras appeared to arise initially as mericlinalor sectorial chimeras, with a proportion of the former ones(5/14) stabilizing into periclinal chimeras. The morphologicalcharacteristics of these latter chimeras were compared withthose of the two parental species. The LII layer of speciesdetermined the characteristics of the vegetative and floralcomponents. However, the LI layer modified the qualitative traitsof both components. The LIII layer on the other hand alteredthe quantitative traits of the vegetative organs and the flower.This layer also determined the growth type, breeding behaviourand inflorescence type of synthesized chimeras. These organizationalfunctions of the LIII layer have not been reported previously. Chimeras, intergeneric chimera synthesis, grafting, graft chimeras, Nicotiana tabacum, Solanum aviculare, p-chlorophenoxyacetic acid, peroxidase isoenzymes