Suppressive effect of lipid A partial structures on lipopolysaccharide or lipid A-induced release of interleukin 1 by human monocytes |
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| Authors: | Ming-Hai Wang Werner Feist Hildegard Herzbeck Helmut Brade Shoichi Kusumoto Ernst Th. Rietschel Hans-Dieter Flad Artur J. Ulmer |
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| Affiliation: | Department of Immunology and Cell Biology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, F.R.G.;Department of Immunochemistry and Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, F.R.G.;Osaka University, Faculty of Science, Toyonaka, Japan |
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| Abstract: | Abstract Experiments were designed to investigate the significance of lipid A partial structures, precursor Ia (compound 406), and lipid X (compound 401) to serve as antagonists of interleukin 1 (IL-1) release from human mononuclear cells and monocytes induced by lipopolysaccharide (LPS, endotoxin) of Salmonella aborus equi or synthetic Escherichia coli lipid A (compound 506). A definite inhibition mediated by lipid A partial structures on IL-1 release induced by LPS or lipid A was found in repeated experiments. The inhibitory effect was exterted not only on IL-1 release, but also on IL-1 peptide synthesis at the intracellular level. The results also show that lipid A partial structures have suppressive effects even when added 1–4 after LPS or lipid A. We conclude from these results that lipis A partial structures (precursor Ia and lipid X) have potent immunomodulatory effects on LPS- and lipid A-induced IL-1 release and may become useful reagents to study the mechanism of interaction of LPS and lipid A with cells of the immune system. |
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| Keywords: | LPS Lipid A Precursor IA IL-1 production |
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