Immunochemical studies on barley seed storage proteins |
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Authors: | Gerald N. Festenstein Frank C. Hay Benjamin J. Miflin Peter R. Shewry |
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Affiliation: | (1) Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ Harpenden, Herts, UK;(2) Immunology Department, Middlesex Hospital Medical School, London, UK |
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Abstract: | The antigenic relationships between the prolamins of barley, rye and wheat have been studied by examining the specificity of an antibody to C hordein in a quantitative study using a laser nephelometer. The antibody reacts weakly with B hordein and strongly with 75-kdalton and 40-kdalton -secalins from rye and 3 some -gliadins from wheat. Absorption experiments and immunodiffusion tests indicate that there are shared antigenic determinants for most of the prolamins. All the species with reacting prolamins belong to the sub-family Festucoideae of the Gramineae. The prolamins of maize, pearl millet and sorghum, species of the sub-family Panicoideae, do not react. The results confirm the known lack of homology between the prolamins of the two sub-families and also indicate the presence of relationships not yet established between C hordein, the 75-kdalton and 40-kdalton -secalins and also 3 gliadin.Abbreviations HMW high molecular weight - PAGE polyacnylamide-gel electrophoresis - PBS phosphate-buffered saline - RLS relative light scattering - SDS sodlum dodecyl sulphate |
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Keywords: | Prolamin Hordein antibody Cereal antigenic relationships |
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