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Methods for cell separation based on sedimentation
Authors:Karen M. K. Lassen  Pedro R. E. Carcia de Caldeano  Claus Emborg
Affiliation:(1) Department of Biotechnology, The Technical University of Denmark, Block 223, DK-2800 Lyngby, Denmark;(2) Department of Food Science and Technology, University of Reading, Whiteknights, RG6 2AP Reading, Berks, England
Abstract:Summary A modified Erlenmeyer flask and a sedimentation chamber have been used for separation of cells from suspension. Preliminary investigations show an efficiency in the separation of more than 80% with yeast cells. Fermentations with the Erlenmeyer flask as separator show a 4–5 times increased cell density for both hybridomas and yeast cells.
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