Intranuclear arrangement of human chromosome 12 correlates to large-scale replication domains |
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Authors: | Masahiro Nogami Orie Nogami Kazuhiro Kagotani Miyako Okumura Hiroshi Taguchi Toshimichi Ikemura Katsuzumi Okumura |
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Institution: | (1) Laboratory of Biological Chemistry, Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu, Mie 514-8507, Japan, JP;(2) Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan, JP |
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Abstract: | The intranuclear arrangement of human chromosome 12 in G0(G1) nuclei from human myeloid leukemia HL60 cells was analyzed by
multicolor fluorescence in situ hybridization (FISH) using band-specific cosmid clones as probes. Pairs of differently colored
cosmids were detected on paraformaldehyde-fixed HL60 nuclei, and their relative positions, internal or peripheral, in individual
nuclei were scored. Our results suggest that the intranuclear arrangement of human chromosome 12 is not random. Some chromosomal
domains, including the centromere, were located in the periphery of the nucleus, while other domains, including the telomeres,
were positioned in the internal areas of the nucleus in G0(G1) cells. Based on the replication banding patterns of metaphase
spreads, human chromosome 12 was divided roughly into five large domains. Interestingly, the clones in late replicating domains
were preferentially localized in the nuclear periphery, whereas clones in early replicating domains were arranged in the internal
areas of the nuclei. The DNA replication timing of each cosmid determined by FISH-based assay did not reflect the replication
bands, but an overall profile of the replication timing was relatively correlated with these domains on chromosome 12. These
results suggest that the intranuclear arrangement of a human chromosome is correlated with the large-scale replication domains,
even before DNA replication.
Received: 23 January 1999; in revised form: 6 September 1999 / Accepted: 11 September 1999 |
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