Antisense knockdown of PKC-alpha using LNA-oligos |
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| Authors: | Hansen Jens Bo Westergaard Majken Thrue Charlotte Albaek Giwercman Birgit Oerum Henrik |
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| Institution: | Cureon A/S, Copenhagen, Denmark. bh@cureon.com |
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| Abstract: | Full-length and 4 nucleotides truncated Locked Nucleic Acid (LNA) modifications of ISIS 3521 were compared for antisense properties in a cellular assay. ISIS 3521 is a 20-mer phosphorothioate designed to hybridise to human protein kinase C-alpha (PKC-alpha) mRNA and is currently submitted to clinical trials against cancer. We report that LNA can potentate this antisense oligo and retain the antisense potential with shorter oligos. |
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