Novel method for the covalent immobilization of oligonucleotides via Diels-Alder bioconjugation |
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| Authors: | Latham-Timmons Hallie A Wolter Andreas Roach J Shawn Giare Rubina Leuck Michael |
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| Institution: | Proligo LLC, Boulder, Colorado 80301, USA. |
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| Abstract: | The synthesis of cyclohexadiene and maleimide derivatives and their use for the functionalization of oligonucleotides and the coating of glass surfaces is reported. A method for the covalent attachment of diene or maleimide modified oligonucleotides to the coated glass surfaces via aqueous Diels-Alder reactions is presented. |
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