Synthesis of 2-deoxy-beta-D-ribose 1-phosphate, NMR comparison and its enzymatic activity for structural elucidation of synthetic alpha-isomer |
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| Authors: | Komatsu Hironori Ikeda Ichirou |
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| Institution: | Catalysis Science Laboratory, Mitsui Chemicals Inc., Sodegaura-shi, Chiba, Japan. hironori.komatsu@mitsui-chem.co.jp |
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| Abstract: | 2-Deoxy-beta-D-ribose 1-phosphate (1) was synthesized in a stereoselective manner and isolated with no detectable contamination by its alpha-isomer (4). Explicit configuration of 4 was first determined by NMR comparison with 1 judging from NOE results and their coupling constants. Natural purine nucleoside phosphorylase (PNPase) did not recognize 1 and gave no products such as alpha- or beta-deoxynucleosides. |
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