Influence of the length of the phosphate chain in mRNA 5' cap analogues on their interaction with eukaryotic initiation factor 4E |
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| Authors: | Zuberek Joanna Jemielity Jacek Niedzwiecka Anna Stepinski Janusz Wyslouch-Cieszynska Aleksandra Stolarski Ryszard Darzynkiewicz Edward |
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| Institution: | Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland. |
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| Abstract: | The recognition of the 5'mRNA cap structure m7G(5')ppp(5')N by one of the components of the initiation translation machinery, the eIF4E factor, plays a pivotal role in regulation of the protein synthesis. In the present study we have shown two opposing roles of the cap phosphate chain in the specific eIF4E-cap interaction. The extension of the phosphate chain enhances the binding of the cap to the unphosphorylated eIF4E but destabilises the eIF4E-cap complex in case of the phosphorylated protein. |
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