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Negatively charged, dye labeled-dideoxynucleotides for "direct-load" DNA sequencing |
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| Authors: | Nampalli Satyam Finn Patrick J Sun Lei Xiao Haiguang Nelson John R Grossmann Gregory Flick Parke K Fuller Carl W Kumar Shiv |
| Affiliation: | Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA. |
| Abstract: | A four-color set of negatively charged, single dye as well as energy transfer dye labeled-ddNTPs were synthesized and evaluated in combination with a novel polymerase in a "direct-load" DNA sequencing, obviating the laborious and time consuming post-reaction work-up. |
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