Novel biotin phosphoramidites with super-long tethering arms |
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Authors: | Morocho A M Karamyshev V N Shcherbinina O V Malykh A G Polushin N N |
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Affiliation: | Fidelity Systems, Inc., Gaithersburg, Maryland 20879, USA. |
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Abstract: | A number of novel biotin phosphoramidites, possessing exceptionally long and uncharged tethering arms, were synthesized from methoxyoxalamido (MOX) and succinimido (SUC) precursors. Included among these monomers is a uridine derivative with the biotin moiety attached through the 2'-position. Some of these phosphoramidites were used to make 5'-biotinylated primers, which were applied in direct sequencing of genomic DNA and capture of Sanger fragment pools. |
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