Pyrazolo[3,4-d]pyrimidine nucleic acids: adjustment of the dA-dT to the dG-dC base pair stability |
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Authors: | He J Becher G Budow S Seela F |
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Institution: | Laboratorium für Organische und Bioorganische Chemie, Institut für Chemie, Universit?t Osnabrück, Osnabrück, Germany. |
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Abstract: | The pyrazolo3,4-d]pyrimidine-4,6-diamine nucleosides 2b-d stabilize the dA-dT base pair significantly when the dA-residue is replaced. Oligonucleotide duplexes incorporating 2b-d show a 4-6 degrees C Tm increase per modification. The 7-bromo compound 2b harmonizes the stability of the dA-dT vs. the dG-dC pair. According to this the stability of such duplexes depends no longer on the base pair composition of a DNA molecule. |
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