Synthesis and energy transfer efficiency of FRET terminators derived from different linkers |
| |
Authors: | Kumar Shiv Nampalli Satyam Finn Patrick J Sudhakar Rao T Chen Chung-Yuan Flick Parke K Fuller Carl W |
| |
Institution: | Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA. shiv.kumar@amersham.com |
| |
Abstract: | A number of different energy transfer dye labeled-cassettes were synthesized using aminoacid based trifunctional linkers and coupled to the propargylamino-substituted dideoxynucleoside-5'-triphosphates (ddNTPs). These terminators were evaluated for their energy transfer efficiency and DNA sequencing potential using thermostable DNA polymerase. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |